Scientist II
Thermo Fisher Scientific
15d ago


Thermo Fisher Scientific is recruiting a Scientist who can foster innovation and antibody development using cutting edge molecular cloning technologies in a high-

  • throughput environment. The recruit must have the ability to troubleshoot and resolve problems in which initiative and judgment are required;
  • the ability to work in a fast-paced environment while maintaining a high level of attention to detail; the ability to organize and present data, the ability to work in a collaborative team environment and to communicate effectively both within the immediate group and across groups


  • Applicants should have a Masters with 5 + years’ experience or a PhD in the area of biochemistry or biophysics integrated with cell biology, molecular biology, immunology or related fields.
  • Experience in various recombinant protein purification methodologies is a must.
  • GLP, GMP or any other certification in laboratory practices and EHS certification will be considered as a bonus
  • Responsibilities

  • Analysis of protein structures (full length or truncated) and modifications in the context of solubility and purification
  • Solubility analysis, sound understanding of primary, secondary and tertiary protein structures
  • Antigen production using various recombinant protein expression methodologies and bacterial, insect or mammalian cell culture systems
  • Selecting appropriate vector, expression systems and purification conditions based on primary literature
  • Select appropriate expression vectors and tags for complex proteins (membrane, toxic proteins etc)
  • Protein purification using multiple purification methodologies (affinity, IEC, SEC etc.)
  • Troubleshooting expression failures on a routine basis and exploring alternate systems to enhance expression and yield
  • Keeping abreast with advancements and next-gen technologies in the protein purification field
  • Be able to work in a high-throughput environment and multitask in order to deliver multiple proteins simultaneously
  • Antibody-antigen affinity analysis using SPR, Biacore, and Octet etc. would be a bonus
  • Responsible for data integrity and accuracy
  • Organizing data reviews and team meetings
  • Other functions such as writing technical protocols and filling out order forms
  • Maintaining inventory of the various raw materials, cDNA constructs and final protein products
  • Deliver high quality results according to project schedule in support of product commercialization during early feasibility, development and validation phases
  • Presenting at national and international conferences
  • Skills

  • Understanding of protein structures and modifications
  • Well-versed in solubility analysis, primary, secondary and tertiary protein structure databases, software and analytics
  • Have extensive experience protein purification and biophysical methods. Experience of working in a high throughput environment is a bonus
  • In depth knowledge of protein expression systems, vectors, tags, chromatography techniques
  • Well-versed with recombinant protein expression and purification methodologies using bacterial, insect and mammalian expression systems
  • Have experience in a high-throughput environment and should have demonstrated multi-tasking abilities for routine purification of proteins at a high scale.
  • Experience in antibody-antigen affinity analysis using Biacore, Octet etc.
  • Well-versed in Structure-function analysis using site-directed mutagenesis or other strategies
  • Should have experience in preparation of competent cells (for use in chemical or electroporation based transformation)
  • GLP experience, documentation and automation
  • Publications in peer-reviewed journals would be a bonus.
  • Good understanding of research projects using standard techniques and protocols
  • Should be a good team player, ready to learn new techniques and innovative thinker
  • Industry experience, bioinformatics and grant writing skills will be considered as a bonus
  • Excellent organizational skills with attention to detail
  • Apply
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